潔凈廠房設(shè)計施工檢測空氣污染步驟
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- 發(fā)布日期：2017-04-13 17:36:43
常用方法步驟如下：
Common methods are as follows:
1．潔凈廠房設(shè)計施工平板檢驗法：用常規(guī)培養(yǎng)基，倒成平板，收入接種室。在事先熏蒸好的接種室，使用前半小時或1小時把供試的培養(yǎng)皿或試管打開，按預(yù)定時間蓋好培養(yǎng)皿或試管。留對照皿不打開。然后一起放入30℃的溫箱中培養(yǎng)48小時，檢查有無菌殖生長，并由菌落形態(tài)，判斷雜菌種類。一般要求平板培養(yǎng)基開蓋5分鐘，菌落不超過3個；敘面培養(yǎng)基開塞 30分鐘者，以不出現(xiàn)菌落為合格。
1 clean workshop design and construction of flat panel test method: using conventional culture medium, pour into plate, income inoculation room. In the first half hour or 1 hours to open the test plate or the test tube to open, cover a good culture dish or a test tube. Leave the control dishes do not open. And then, 48 hours, 30 hours, the culture of sterile growth, and by the colony morphology, determine the type of miscellaneous bacteria. General requirements for plate culture Jikaigai 5 minutes and colony does not exceed 3; Syrian surface medium glycerine 30 minutes, to does not appear to be a colony for qualified.
2．潔凈廠房設(shè)計施工 http://www.jnjkjh.com/ 斜面檢驗法：將常用的瓊脂斜面培養(yǎng)基各取二管， 放入接種室，按無菌操作各將其中的一管的棉塞取出，放在滅菌的培養(yǎng)皿內(nèi)。經(jīng)過一定時間后，再將棉塞通過火焰，塞回試管，連同對照一起培養(yǎng)。經(jīng)48小時后，檢驗有無雜菌生長。和上邊方法同。
2 clean workshop design and construction method: the commonly used agar slant medium each take two tube, into the inoculation room, according to the aseptic operation each will be one of the tube of the cotton plug out, placed in the sterile culture dish. After a certain period of time, then the cotton plug through the flame, plug back into the test tube, along with the control to develop. After 48 hours, the growth of the bacteria was tested. And the above method.
3．潔凈廠房設(shè)計施工空氣污染情況檢驗：在接種過程中，人員的出過、器材的搬放、接種操作的動作等原因造成空氣污染，檢驗方法是在準(zhǔn)備工作結(jié)束后，接種操作開始時，按上述方法打開培養(yǎng)皿蓋子或試管塞，經(jīng)5分鐘、30分鐘或直到工作結(jié)束時再蓋好，以檢驗在不同的使用時間內(nèi)，空氣污染的程度。
3 clean plant design and construction of air pollution: in the course of vaccination, personnel, equipment, equipment, operation and other reasons to cause air pollution, test method is in the preparation of the work, after the start of the operation, according to the above method to open the lid or test tube plug, 5 minutes, 30 minutes or until the end of the work, to test the extent of air pollution in different time.
如霉菌較多時；可先用5％石炭酸全面噴射后，再用甲醛熏蒸。如細菌較多時，可采取用乳酸和甲醛交替熏蒸的辦法加以殺滅。
Such as mould more; first with 5% overall after spraying with phenol, formaldehyde fumigation. If the bacteria are more, can take the method of alternating with lactic acid and formaldehyde to kill.
